TY  - THES
N2  - ABSTRACT
Autoimmune or ?lupus? is immune disorders caused by various factors, one of which is 
Streptococcus pyogenes bacteria. S. pyogenes bacteria activity can be suppressed using 
corticosteroid and antibiotic therapy, but has side effects. Tapak Dara (Catharanthus roseus
`(L.) G. Don.) leaves known to have potency as an alternative antibacterial derived from 
secondary metabolites, namely 70 types of alkaloids in the form Triterpenoid Indol Alkaloids 
(TIAs), flavonoids, saponins, steroids, tannins, phenolics, antioxidants, and a little of essential 
oil. The purpose of this research are to determine the optimum concentration of 96% ethanolic 
and 96% methanolic extract from tapak dara leaves and the type of solvent extract which has the 
best antibacterial activity. Antibacterial potency test was carried out with dilution method to 
find KHM and diffusion to find DDH which in triplo at 96% ethanolic extract concentration of 
45%, 55%, 65%, and 75%, while at methanolic 96% extract concentration of 15%, 25%, 35%, 
and 45%. The control was used DMSO 10% for negative control and 10 mg/L Amikacin 
antibiotic for positive control. Phytochemical tested with extract characterization and statistical 
test using Anova with ?=0,05. Based are the results of the research, optimum concentration to 
inhibit S. pyogenes bacteria growth are concentration of 55% ethanolic extract with average 
diameter of inhibition area is equal to 9,20 ±0,05 mm and concentration of 45% methanolic 
extract with average diameter of inhibition area is equal to 19,583±0,803 mm. As for the solvent 
which has the best antibacterial activity is the methanolic solvent. 
Keywords: Antibacterial, Catharanthus roseus, Streptococcus pyogenes
A1  - Artiningsih Sayekti, Nita
A1  - Triastinurmiatiningsih
A1  - Komala, Oom
Y1  - 2019/07//
UR  - http://eprints.unpak.ac.id/3022/
PB  - Universitas Pakuan
TI  - Aktivitas Ekstrak Etanol Dan Metanol Daun Tapak Dara (Catharanthus roseus
(L.) G. Don.) sebagai Antibakteri Streptococcus pyogenes
ID  - eprintsunpak3022
AV  - none
M1  - Skripsi
ER  -