%I Universitas Pakuan
%D 2024
%L eprintsunpak7669
%A Novel Novel
%A Leny Heliawati
%A Siti Warnasih
%X KTIVITAS ANTIOKSIDAN EKSTRAK DAUN KETAPANG 
(Terminalia cattapa L.) DENGAN BERBAGAI METODE DAN UJI 
SITOTOKSIKNYA
Novel Suci Nurania)
, Leny Heliawati a), Siti Warnasih a*)
a) Department of Chemistry, Pakuan University, Bogor
*) Corresponding Author: sitiwarnasih@unpak.ac.id
ABSTRACT
Ketapang leaves (Terminalia catappa L.) are wild plants that contain secondary metabolite 
compounds, namely flavonoids, triterpenoids, and tannins that have potential as antioxidants. 
Flavonoid metabolite compounds in ketapang leaves are thought to have anticancer activity and 
reduce cell viability. This study aims to determine the antioxidant and cytotoxic activity of methanol 
extracts of ketapang leaves (Terminalia catappa L.) and identify secondary metabolite compounds 
using GC-MS, which later can have potential as anticancer. The study began with maceration 
extraction using 96% methanol then fractionated with n-Hexan and Ethyl Acetate. Antioxidant 
activity was tested using DPPH, CUPRAC and FRAP methods. Furthermore, the best antioxidant 
activity value from the three methods was then tested for cytotoxicity using the BSLT (Brine Shrimp 
Lethality Test) method and identified active compounds using GC-MS. The results showed that 
methanol extract, N-Hexan fraction and Ethyl acetate fraction of ketapang leaves gave the highest 
antioxidant activity using DPPH, CUPRAC and FRAP methods with the best results with levels of 
18.2855; 302.7199; and 180.4672 µg vitamin C/mg extract in methanol extract. The results of 
probit analysis in the cytotoxic test showed that the methanol extract had an LC50 value of 410.96 
ppm so that the methanol extract was toxic. There are 23 compounds based on GC-MS tests on 
methanol extracts, one of which is epicatechin, Epigallocationchin gallate, and piperidine 
compounds which have potential as anticancer.
Keywords: Ketapang Leaf (Terminalia Catappa L), Antioxidant, Cytotoxi, and GC-MS
%T KTIVITAS ANTIOKSIDAN EKSTRAK DAUN KETAPANG 
(Terminalia cattapa L.) DENGAN BERBAGAI METODE DAN UJI 
SITOTOKSIKNYA