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Penentuan Kadar KuersetinBunga Talang (clitoria ternate L.) Secara Kromatografi Cair Kinerja Tinggi Berdasarkan Perbedaan Metode Ekstrak
Comparison of Maceration and Ultrasound Assisted Extraction Method in Determining Quercetin Level Clitoria ternatea L. Flowers Sri Wardatun1*, Akmal Zainul Ahyar2 , Euis Nining3 1,2,3Department of Pharmacy, Universitas Pakuan, Bogor, Indonesia sri.wardatun@unpak.ac.id https://doi.org/10.33751/jf.vxxix.xxxx Article info: Submitted : D-M-202X Accepted : D-M-202X Published : D-M-202X This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License Copyright © 2023 Authors Published by: Universitas Pakuan ABSTRACT Butterfly pea flowers (Clitoria ternatea L.) contain the compound quercetin which has been studied extensively. Butterfly pea flowers have shown various pharmacological activities. The extraction method have an impact on the amount of active compound extracted. The aim of this research was to determine the effect of different extraction methods on quercetin levels in C. ternatea L flowers using the High Performance Liquid Chromatography (HPLC) method. The HPLC system uses an ODS-3 column stationary phase; a mobile phase mixture of 0.1 % orthophosphoric acid and methanol (36:64); a flow rate of 1.2 mL/min; and a PDA-UV detector. The extraction methods used were maceration, and ultrasound-assisted extraction (UAE) using methanol as a solvent. The results showed that the quercetin content of C. ternatea L flower extract with maceration method results was 1.0669 ± 0.0283 mg/g and with UAE method was 1.3915 ± 0.1789 mg/g. Statistical test results showed that differences in extraction methods did not have a significant effect on quercetin levels. However, the UAE method is considered more efficient in terms of extraction time, so the UAE method can be used as an alternative for extracting quercetin from C. ternatea L. flowers. Keywords: C. ternatea L.; flowers; maceration; UAE; quercetin INTRODUCTION Clitoria ternatea L. flowers are widely used in traditional medicine. This part has been used to treat several diseases. The pharmacological activities of C. ternatea L. flowers extract were antidiabetic, antioxidant, antimicrobial, anti-inflammatory, anti-arthritic, analgesic, and cytotoxic (Jeyaraj et al., 2022; Shinde & Khemnar, 2024). C. ternatea L. flowers have striking colors, so they are often used as a natural food and drink coloring (Gamage et al., 2021; Rahma et al., 2024). The ethanol extract of C. ternatea L. flowers contain alkaloids, flavonoids, resins, tannins, polyphenols, saponins, and triterpenoids (Rahma et al., 2024; Jayanti et al., 2021; Sarma et al., 2023; Yee & Than, 2020). The widely known active compound in C. ternatea is quercetin. According to Jeyarai et al. (2020), phytochemical compounds that have been isolated from C. ternatea L. flowers were quercetin and myricetin glycosides known as anthocyanins. Furthermore, quercetin 3-2G�rhamnosylrutinoside, quercetin 3-neohesperidoside, quercetin 3-rutinoside, quercetin 3- glucoside, myricetin 3-neohesperidoside, myricetin 3-rutinoside, and myricetin 3-glucoside were found in C. ternatea L. flowers (Al-Snafi et al., 2016; Mahajan et al., 2022). Quercetin is one of the natural bioactive products from the flavonol family, which is widely studied because it has pharmacological activities such as anticancer, anti-inflammatory, anti-obesity, preventing
Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan
Fakultas Matematika dan Ilmu Pengetahuan Alam
Penentuan Kadar KuersetinBunga Talang (clitoria ternate L.) Secara Kromatografi Cair Kinerja Tinggi Berdasarkan Perbedaan Metode Ekstrak (Text)
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