<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Hydrolytic and Transglycolation Characteristics of Xanthomonas\r\ncampestris and Bacillus megaterium in Several Substrates"^^ . "Hydrolytic and Transglycolation Characteristics of Xanthomonas\r\ncampestris and Bacillus megaterium in Several Substrates\r\nHeny Herawatia,*, Elmi Kamsiatia, Sunarmania\r\n, S. Joni Munarsoa\r\n, Ina Supriyatnab\r\n, Tri Aminingsihb\r\na\r\nIndonesia Center for Agricultural Postharvest Research and Development, Bogor 16111, West Java, Indonesia\r\nbPakuan University, MIPA 1 Building, Bogor 16143, West Java, Indonesia\r\nCorresponding author: *\r\nherawati_heny@yahoo.com\r\nAbstract— Some microbes can produce hydrolytic enzymes and have transglycosylation capacity at the same time, including\r\nXanthomonas campestris and Bacillus megaterium. The enzyme characteristics of the microbes can be observed from their activity by\r\nusing several types of substrates. This research aimed to characterize the hydrolytic and transglycosylated CGTase enzyme activity\r\nfrom Xanthomonas campestris and Bacillus megaterium on glucose, tapioca, and corn starch media at several concentrations. Stages of\r\nresearch included bacterial rejuvenation, growth on the substrate, and analysis (hydrolytic activity, carbohydrate concentration, and\r\ntransglycosylation activity). The research design used was a Completely Randomised Design (CRD). The data from observations were\r\nthen analyzed using Analysis of Variance (ANOVA) with the Duncan Multiple Range Test (DMRT) at a 5% level to determine the\r\neffect of treatment on all observational variables. Data analysis was carried out using the SPSS Statistics 17.0 program. The analysis\r\nresults showed Xanthomonas campestris and Bacillus megaterium with tapioca and cornstarch substrates of 2%, 4%, and 6%,\r\nrespectively, had hydrolytic activity. CGTase enzymes produced from Xanthomonas campestris and Bacillus megaterium with glucose,\r\ntapioca, and corn starch substrates 2%, 4%, and 6%, had intramolecular transglycosylation activity. The CGTase hydrolytic activity\r\ntest's analysis showed that the CGTase hydrolytic test's significance value was significantly different between treatments with an\r\nincubation time of 24 to 120 hours. The substrate, a carbon source, greatly influences the rate of enzyme production produced by\r\nmicroorganisms.\r\nKeywords— Substrate; hydrolytic; transglycosylation; Xanthomonas campestris; Bacillus megaterium."^^ . "2019-09-29" . . "Universitas Pakuan"^^ . . . "Fakultas Matematika dan Pengetahuan Alam, Universitas Pakuan"^^ . . . . . . . . . . . "Heny"^^ . "Herawati"^^ . "Heny Herawati"^^ . . "Tri"^^ . "Aminingsih"^^ . "Tri Aminingsih"^^ . . "Ina"^^ . "Supriyatna"^^ . "Ina Supriyatna"^^ . . "Universitas Pakuan"^^ . . . "Fakultas Matematika dan Ilmu Pnegetahuan Alam"^^ . . . "Program Studi Kimia"^^ . . . . . . "HTML Summary of #10099 \n\nHydrolytic and Transglycolation Characteristics of Xanthomonas \ncampestris and Bacillus megaterium in Several Substrates\n\n" . "text/html" . . . "Kimia"@en . .